BROAD RANGE PCR-BASED COMPOSITIONS AND METHODS FOR

专利名称：BROAD RANGE PCR-BASED COMPOSITIONS

AND METHODS FOR THE DETECTION ANDIDENTIFICATION OF FUNGAL PATHOGENS

发明人：David N. Fredricks,Prasanna D. Khot,Daisy L.

Ko

申请号：US12625361申请日：20091124

公开号：US20100129821A1公开日：20100527

专利附图：

摘要：Disclosed herein are methods for detecting a fungal pathogen in a patient

sample, involving isolating the sample, carrying out a PCR reaction on the sample togenerate an amplicon that includes a region of the fungal 28S ribosomal RNA gene, anddetecting the PCR amplicon. Also disclosed are sequences of primers for specificallydetecting a broad range of fungal pathogens in the presence of human ribosomal DNA.In certain embodiments, the amplicon is detected by sequencing or by two-dimensionalmelt-curve analysis. In yet other embodiments, more than one fungal pathogen isdetected in a sample using the methods disclosed herein.

申请人：David N. Fredricks,Prasanna D. Khot,Daisy L. Ko

地址：Seattle WA US,Mountlake Terrace WA US,Seattle WA US

国籍：US,US,US

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