Overview
Ocean Optics USB2000, USB2000+, USB4000, HR2000, HR2000+, HR4000, Jaz, Maya, QE65000, NIR Series, NIRQuest Series, S2000, and S1024DW Spectrometers provide several methods of acquiring data (see table below). In the Normal mode, the spectrometer is “free running.” That is, the spectrometer is continuously scanning, acquiring, and transferring data to your computer, according to parameters set in the software. In this mode, however, there is no way to synchronize the scanning, acquisition, and transfer of data with an external event. However, trigger pulses for synchronizing an external event with the spectrometer are available.
To synchronize data acquisition with external events, other modes of acquiring data are available. Each mode involves connecting an external triggering device to the spectrometer and then applying an external trigger to the spectrometer before the software receives the data. The length of the integration time and the source for the integration clock depend upon the mode chosen. All other acquisition parameters are set in the software.
For spectrometers with FPGA firmware versions of 3.0 or higher, and for spectrometers not covered by this document, see New External Triggering Options Instructions for Spectrometers with Firmware Version 3.0 and Above. Also see the HR4000 and USB4000 Shutter Mode Performance in Hardware Trigger Mode for more information on external triggering with these spectrometers. Triggering Mode
Normal External Software
Description
Spectrometer acquires spectra continuously.
Integration time is set in the software. Software
receives a trigger event and transmits spectra obtained in the data acquisition cycle in which the trigger occurred.
Integration time set via chip in spectrometer (USB Series/Jaz/HR Series/S2000 D Series) or jumpers (S2000 series). Spectrometer waits for a sharp rise in voltage on the trigger input pin, and then acquires spectra until the voltage is removed.
Spectrometer acquires data from an external trigger event (such as a push button) until the next time the trigger is activated, at which time the spectrometer ceases spectral acquisition and begins a new
acquisition. Integration time cannot be set, since the trigger can fire at random intervals.
Applicable Spectrometer(s)
All All
External Hardware
USB2000/USB4000/Jaz/ HR2000/HR2000+/HR4000/ S2000/S1024DW
External
Synchronization
S2000/S1024DW
200-00000-000-01-201401 1
External Triggering Options Instructions
Triggering Mode Description Applicable Spectrometer(s)
Quasi-Real Time The integration clock is set to 4ms, which is the time QE65000, Maya2000, Acquisition required to completely read out all the pixels. When the Maya2000 Pro Mode software (user) requests a spectrum, the integration
clock waits for the current period to expire, changes to the desired integration time set via software commands, and then returns to 4ms. With this
approach, the start of the integration period will always be within 4ms of the time when the request for a spectrum is issued. No trigger signal is required to operate in this mode.
IMPORTANT: Voltage Regulation
The maximum recommended voltage on the Triggering Pin is 5.5 V. If your triggering device exceeds this voltage, you must regulate or condition the signal (via transistor buffering, transformer isolation or opto-isolation, for example) or isolate the signal from the spectrometer.
Note
To use one of the External Triggering options, you must know the specifications and limitations of the triggering device. The design of the triggering device may prevent you from using one of the external triggering modes as it is described in these pages.
Consult the following images and table for information on which pin to connect to the triggering device when using each triggering mode on the spectrometer.
Pinout Diagrams for Ocean Optics Spectrometers
J2 (D-SUB-15) Accessory Connector on NIR Series, S2000 and S1024DW Spectrometers
Pinout Diagram of 10-pin Connector on USB2000 Spectrometer
Pinout Diagram of 22-pin Connector on USB2000+, USB4000, Jaz and HR2000 Spectrometers (be sure to also use Ground Pin 6 when triggering)
Pinout Diagram of 30-pin Connector on HR2000+, HR4000, JazNIRQuest Series, and QE65000 Spectrometers
2 200-00000-000-01-201401
External Triggering Instructions
Trigger Input Pin Information for Ocean Optics Spectrometers
Trigger Input Pin
Trigger Mode
S2000 S1024DW
QE65000
USB2000 USB2000+ USB4000 HR2000 Jaz
HR4000 HR2000+
NIR Series NIRQuest Series
Description
External Software
8
10 7
10 10
Asynchronous Mode -
Integration time specified in software
Synchronous Mode - User provides integration clock Synchronous Mode -
Integration fixed by jumpers
External
5
Synchronization External Hardware
4
Not Not Not Not
Available Available Available Available Not
7
Available
10
Not
Available
External Triggering vs. Triggering an External Event
There could be some confusion between the concepts of External Triggering and triggering an external event. The following sections explain each of these concepts.
External Triggering
In External Triggering, an event outside the sampling system (such as a push button, lever activation, or laser pulse) triggers the voltage level on the spectrometer’s trigger pin and instructs the spectrometer to begin spectra acquisition.
Triggering an External Event
When triggering an external event, the spectrometer instructs an external device (typically a lamp such as the PX-2 or the LS-450) to illuminate immediately prior to spectral acquisition.
200-00000-000-01-201401 3
External Triggering Options Instructions
Level Triggering and Edge Triggering
In External Triggering, there are two modes of triggering available. The trigger mode in use determines the kind of triggering that the system will use.
Level Triggering
Level triggering mode is only available when using External Software Triggering mode. In this mode, the software monitors the A/D converter for an increase in the voltage level applied to the trigger pin. When the appropriate increase in voltage is received (5 volts), the software instructs the spectrometer to acquire spectra.
In this mode, the A/D converter will acquire data in intervals based on the integration time set in the software. Thus, if a trigger occurs in the middle of an integration period, the software will retrieve the spectra obtained for that entire integration period (before AND after the trigger event). Similarly, if a trigger occurs immediately prior to the end of an integration period, the software will still retrieve the spectra obtained during that integration period.
For example, if the integration time is 50 ms and the trigger event occurs 48 ms into the integration cycle, the 2 ms following the trigger will be acquired, and the 48 ms prior to and including the trigger will be acquired.
Edge Triggering
Edge triggering mode is available when using External Hardware and External Synchronization
Triggering modes. In both of these modes, the spectrometer monitors the trigger input pin for rapid rise in the voltage (which may or may not reach the 5 volt plateau) applied to the trigger pin. When this voltage rise is received and the spectrometer “sees” the voltage peak caused by the increase, the spectrometer acquires spectra and transmits the spectra to the software.
In External Hardware Triggering mode, the spectrometer begins an acquisition upon receiving trigger and acquires for one integration cycle. In External Synchronization Triggering mode, however, data acquisition continues until the next rise in voltage is “seen” by the spectrometer.
4 200-00000-000-01-201401
External Triggering Instructions
Integration Times Available with External Triggering
The following table illustrates the integration times available with External Triggering modes: Spectrometer
USB2000/HR2000
External Software
Set in Software
External Hardware
50 msec
External
Synchronization
Cannot Manually Set
Input by software Not Available Not Available Cannot Manually Set
Cannot Manually Set
Not Available
USB4000/USB2000+/Jaz/HR2000+/HR4000 Set in Software NIR Series NIRQuest Series S2000/S1024DW
Set in Software Set in Software Set in Software
Set in Software Not Available Not Available Set on Jumpers
S2000 D-Series Set in Software 55 msec
QE65000 Set in Software Not Available
Software Support
The following table details External Triggering support capacity in all Ocean Optics software products: Product
SpectraSuite OOIBase OOIBase32 OOIBase32 Platinum OOIColor
OOI Driver Products
Supported
All Modes All Modes All Modes All Modes
External Software and External Hardware All Modes
Not Supported
-- -- -- --
External Synchronization --
200-00000-000-01-201401 5
External Triggering Options Instructions
Product
OOISensors OOIIrrad Palm Spec
Supported
External Software
External Software and External Hardware External Software
Not Supported
External Synchronization and External Hardware
External Synchronization
External Sync and External Hardware
Configuring External Triggering
External Software Trigger Mode
In this level-triggered mode, the spectrometer is “free running,” just as it is in the Normal mode. The spectrometer is continually scanning and collecting data. With each trigger, the data collected up to the trigger event is transferred to the software. If you continuously apply triggers (for example, by holding down the button on via an external switch), this mode is equivalent to operating in the Normal mode. In the External Software Trigger mode, you set the integration time (as well as all other acquisition parameters) in the software. The source for the integration clock comes from the A/D converter.
If the software trigger is asserted during integration cycle n, the photons from this integration period will be read out and digitized at the start of
integration cycle n+1
External Software Triggering – Trigger Timing
When to Use the External Software Trigger Mode
Use the External Software Trigger mode if you:
• Are using a continuous illumination source, and the light intensity is constant before, during, and
after the trigger.
• Need to set the integration time in the software.
6 200-00000-000-01-201401
External Triggering Instructions
Using the External Software Trigger Mode
► Procedure
1. Connect the line to the appropriate pin on the spectrometer:
USB2000, USB2000+, USB4000, Jaz, and HR2000 Spectrometers Supply a line from Pin 7 of the Multi-pin Connector on the spectrometer to your triggering device.
HR2000+, HR4000 and QE65000 Spectrometers Supply a line from Pin 10 of the Multi-pin Connector on the spectrometer to your triggering device.
NIR Series, S2000, and S1024DW Spectrometers Supply a line from Pin 8 of the J2 Accessory Connector to the triggering device, and then connect Pin 10 of the J2 Accessory Connector (ground) to the ground of the triggering device. 2. Set triggering in the software:
SpectraSuite Use the External Trigger selection box on the Trigger toolbar to set the trigger mode of the spectrometer to Software.
OOIBase32 a. b. c.
Click the Data Acquisition icon or select Spectrum | Configure Data Acquisition from the OOIBase32 menu bar and set the acquisition parameters in the software
Select the External Trigger page on the Configure Data Acquisition screen and select Software Trigger.
Enable the Automatically save file on trigger box if you wish to save the data to a file after each trigger.
If you enable this function, OOIBase32 displays a file save dialog box after each trigger. To avoid manually naming a file for each trigger, you can enable the Autoincrement Filenames function from the File | Autoincrement Filenames | Enabled option in the menu bar.
Note
200-00000-000-01-201401
7
External Triggering Options Instructions
Once you select an external trigger mode, your computer will appear unresponsive. This is normal, as the computer is waiting for a trigger. You must apply one more trigger to the spectrometer after selecting a new trigger mode.
Since this is a level-triggered mode, the delay between the trigger pulse and spectrum acquisition varies, depending on the speed at which the software polls the line and recognizes it is HIGH, as well as the amount of time until the start of the next integration period.
External Synchronization Trigger Mode – S2000 and S1024DW Spectrometers Only
In this edge-triggered mode, the spectrometer is idle until you apply the initial trigger to the spectrometer. With each trigger, the spectrometer:
1. 2. 3. 4.
Stops taking its current scan (if applicable) Transfers data to the software Begins a new scan
Integrates until another trigger is applied
In the External Synchronization Trigger mode, the frequency of triggers to the spectrometer determines the effective integration time. The effective integration time is the time between rising edges of signals applied to Pin 5 of the J2 Accessory Connector. All other acquisition parameters are set in the software. The source for the integration clock is the external triggering device itself, such as a periodic TTL signal.
External Synchronization Triggering – Trigger Timing
When to Use External Synchronization Trigger Mode
Use the External Synchronization Trigger mode if you:
• Must synchronize your scans to an external clock source • Are using a lock-in amplifier • Are using a chopper
8 200-00000-000-01-201401
External Triggering Instructions
Using the External Synchronization Trigger Mode
► Procedure
1. Supply a line from Pin 5 of the J2 Accessory Connector to the triggering device. (See figure in
Pinout Diagrams for Ocean Optics Spectrometers on page 2 for pin location.) 2. Connect Pin 10 of the J2 Accessory Connector (ground) to the ground of the triggering device. 3. Click the Data Acquisition icon or select Spectrum | Configure Data Acquisition from the
OOIBase32 menu bar and set the other acquisition parameters. 4. Select the External Trigger page on the Configure Data Acquisition screen and select
Synchronization.
5. Enable the Automatically save file on trigger box if you wish to save the data to a file after each
trigger.
If you enable this function, OOIBase32 displays a file save dialog box after each trigger. To avoid manually naming a file for each trigger, you can enable the Autoincrement Filenames function from the File | Autoincrement Filenames | Enabled option in the menu bar.
Note
Once you select an external trigger mode, your computer will appear unresponsive. This is normal, as the computer is waiting for a trigger.
External Hardware Trigger Mode – USB2000, USB2000+, USB4000, Jaz, HR2000, HR2000+ and HR4000 Spectrometers
In this edge-triggered mode, the spectrometer is idle until you apply a trigger to the spectrometer. With each trigger, the following events occur:
1. The spectrometer is cleared and then begins integrating
2. For USB2000 and HR2000: The spectrometer integrates for 50 milliseconds (you can contact
Ocean Optics if you require a different integration time)
For USB2000+, USB4000, Jaz, HR2000+ and HR4000: The integration time is selectable in software
3. The data is transferred to the software; the spectrometer then sits idle waiting for the next trigger
200-00000-000-01-201401 9
External Triggering Options Instructions
External Hardware Triggering – Trigger Timing
When to Use External Hardware Triggering Mode
Use the External Hardware Triggering mode if you:
• Are using a pulsed excitation source or light source in your experiment (such as a laser or flash
lamp)
• Are doing LIF (fluorescence with pulsed excitation) or phosphorescence experiments • Need to synchronize an acquisition with an external event
Using External Hardware Triggering Mode
Follow the steps below to enable External Hardware Triggering mode.
► Procedure
1. Supply a line from Pin 7 on the USB2000/USB2000+/Jaz//HR2000/USB4000 or Pin 10 on the
HR2000+/HR4000 of the Multi-pin Connector to your triggering device. 2. Set your acquisition parameters, such as integration time, in the software.
SpectraSuite Use the External Trigger selection box on the Trigger toolbar to set the trigger mode of the spectrometer to External Hardware.
OOIBase32 a. Select Spectrum | Configure Data Acquisition from the menu. Choose the External Trigger
tab and select Hardware Trigger. b. Enable the Automatically save file on trigger box to save processed data with each external
trigger.
10 200-00000-000-01-201401
External Triggering Instructions
If you enable this function, you will be presented with a file save dialog box with each trigger.
To avoid manually naming a file for each trigger, you can enable the Autoincrement
Filenames function by selecting File | Autoincrement Filenames | Enabled from the menu. Choose a base name and starting index for the autoincremented files.
Note
Once you select the External Hardware Trigger mode, your system may appear unresponsive. However, the system is actually waiting for the trigger.
c. Activate your triggering device. The acquisition parameters, name of the window acquiring
data and trigger mode are displayed in the main status bar.
External Hardware Trigger Mode – S2000 and S1024DW Spectrometers
In this edge-triggered mode, the spectrometer is idle until you apply a trigger to the spectrometer. With each trigger, the spectrometer:
1. Clears its memory and then begins integrating
2. Integrates for a fixed period of time, determined by the jumpered pins on the spectrometer’s
circuit board
3. Transfers the data to the software 4. Waits for the next trigger
In the External Hardware Trigger mode, you set the integration time by positioning jumpers over pins on the spectrometer’s circuit board. All other acquisition parameters are set in the software. The source for the integration clock is the external triggering device itself. This mode is ideal for use with laser and other short-pulse events.
External Hardware Triggering – Trigger Timing
200-00000-000-01-201401 11
External Triggering Options Instructions
When to Use External Hardware Trigger Mode
Use the External Hardware Trigger mode if you:
• • • •
Are using a pulsed excitation or light source in your experiment
Are doing LIF (fluorescence with pulsed excitation) or phosphorescence experiments Are able to jumper the pins on Jumper Block 4 of the spectrometer’s electronic board Need to synchronize an acquisition with an external event
Using the External Hardware Triggering Mode
► Procedure
Supply a line from Pin 4 of the J2 Accessory Connector to the triggering device. (See figure in Pinout Diagrams for Ocean Optics Spectrometers on page 2 for pin location.)
1. Connect Pin 10 of the J2 Accessory Connector (ground) to the ground of the triggering
device. 2. Consult the charts below to determine the integration time. Identify the Spectrometer and
A/D converter you are using, and then choose the integration time.
S2000 Miniature Fiber Optic Spectrometer
Integration Time ADC500, SAD500 (500 kHz Master Clock Freq)
8.8 ms 27.5 ms 55 ms 137.5 ms
ADC1000
(1 MHz Master Clock Freq)
4.4 ms 13.3 ms 27.5 ms 68.8 ms
ADC2000
(2 MHz Master Clock Freq)
2.2 ms 6.7 ms 13.8 ms 34.4 ms
Integration Time Equation
Jumper Block 4 (JP4)
Pins 1-2 Pins 3-4
8.8/Fm 27.5/Fm 55/Fm 137.5/Fm
open open shorted shorted
open shorted open shorted
Fm = the master clock frequency in MHz; shorted = connected; open = disconnected
12 200-00000-000-01-201401
External Triggering Instructions
S1024DW Miniature Fiber Optic Spectrometer
Integration Time SAD500
(500 kHz Master Clock Freq)
5 ms 60 ms 300 ms 655 ms
Jumper Block 4 (JP4)
Integration Time Equation
Pins 1-2
open open shorted shorted
Pins 3-4
open shorted open shorted
1000/Fm 12000/Fm 60000/ Fm 131070/ Fm
Fm = the master clock frequency in Hz (i.e., 200 kHz for the SAD500); shorted = connected; open = disconnected
► Procedure
1. Note the configuration of the pins in the Jumper Block 4 column of the chart. 2. Remove the spectrometer from its housing. Do not tamper with the optical bench.
(If you have more than one channel in your system, you may have to disconnect the channels from one another. The master spectrometer is always on the bottom of a multiple channel system.) 3. Locate Jumper Block 4 (JP4) in the center of the green circuit board, near the
optical bench. (See the figure to the right.) Jumper Block 4 consists of the first four pins, which are numbered 1, 2, 3, and 4. 4. Using jumpers, configure the pins to match the integration time you selected.
For example, if you have an S2000 and an ADC1000 A/D Converter, you select
13.3 milliseconds as the integration time. To enable this, place a jumper over pins 3 and 4 to short the pins, and leave pins 1 and 2 open.
5. Click on the Data Acquisition icon or select Spectrum | Configure Data Acquisition from the
OOIBase32 menu bar, then set other acquisition parameters (averaging, boxcar smoothing, etc.) in the software. 6. Select the External Trigger page from the Configure Data Acquisition screen, and then select
Hardware Trigger.
200-00000-000-01-201401 13
External Triggering Options Instructions
7. Enable the Automatically save file on trigger box if you wish to save the data to a file after each
trigger.
If you enable this function, OOIBase32 displays a file save dialog box after each trigger. To avoid manually naming a file for each trigger, you can enable the Autoincrement Filenames function from the File | Autoincrement Filenames | Enabled option in the menu bar.
Note
Once you select an external trigger mode, your computer will appear unresponsive. This is normal, as the computer is waiting for a trigger.
It is important to note that if you apply triggers faster than the fixed integration time, you will miss acquisitions.
Cables
To configure external triggering functions with an Ocean Optics spectrometer, you may need one of the following cables. To order any of these cables, contact an Ocean Optics Applications Sales Engineer.
14 200-00000-000-01-201401
因篇幅问题不能全部显示，请点此查看更多更全内容